redivue alpha 32p dctp (Amersham Life Sciences Inc)
86
Structured Review
Amersham Life Sciences Inc
redivue alpha 32p dctp
Redivue Alpha 32p Dctp, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+32p+dctp/32p+a+dctp/us12655395-1473-11-14
Average 86 stars, based on 1 article reviews
Redivue Alpha 32p Dctp, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+32p+dctp/32p+a+dctp/us12655395-1473-11-14
Average 86 stars, based on 1 article reviews
redivue alpha 32p dctp - by Bioz Stars,
2026-10
86/100 stars
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Synthesized:Article Title: Decysin, a new member of the metalloproteinase family, is regulated by prolactin and steroids during mouse pregnancy. Article Snippet: .. Probes were synthesized from specific cDNA (25 ng) using the Rediprime II kit and [ Reverse Transcription:Article Title: A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male development. Article Snippet: After annealing, the DNA duplexes were de-salted through a Sephadex G-50 spin column (GE healthcare, 27-5330-01). .. For radioactive labelling, a 5’ overhang G at both ends of probes was used to fill in with Article Title: Changes in microvessel endothelial cell gene expression in an in vitro human breast tumour endothelial cell model. Article Snippet: Selective targeting of tumour-endothelium has been proposed as a means of therapy.. The successful exploitation of this approach will rely on the identi®cation of suitable targets expressed speci®cally on the tumour-associated endothelium.. In an attempt to identify novel tumour-endothelium associated targets we have used di erential mRNA display to identify genes up-regulated in an in vitro breast tumour-endothelial cell culture model. Con ̄uent monolayers of human mammary microvessel endothelial cells (HuMMEC) were incubated for 5 days with MDAMB-231 breast adenocarcinoma cell-conditioned medium (TCM). mRNAs isolated from TCM-treated and control cells were ampli®ed using 104 combinations of four 30 anchored T12VN primers and 26 `random' 10mers by RTPCR and the products examined on DNA sequencing gels. DNA Labeling:Article Title: Essential role of c-Jun induction and coactivator p300 in epidermal growth factor-induced gene expression of cyclooxygenase-2 in human epidermoid carcinoma A431 cells Article Snippet: Cyclooxygenase-2 (COX-2) is an inducible enzyme responsible for high-level prostaglandin production during inflammation and carcinogenesis.. In this study, the transcriptional regulation of COX-2 expression induced by epidermal growth factor (EGF) in human epidermoid carcinoma A431 cells was studied.. EGF treatment induced the expression of COX-2 mRNA, protein, promoter and enzyme activity in a time-dependent manner. cDNA Synthesis:Article Title: Changes in microvessel endothelial cell gene expression in an in vitro human breast tumour endothelial cell model. Article Snippet: Selective targeting of tumour-endothelium has been proposed as a means of therapy.. The successful exploitation of this approach will rely on the identi®cation of suitable targets expressed speci®cally on the tumour-associated endothelium.. In an attempt to identify novel tumour-endothelium associated targets we have used di erential mRNA display to identify genes up-regulated in an in vitro breast tumour-endothelial cell culture model. Con ̄uent monolayers of human mammary microvessel endothelial cells (HuMMEC) were incubated for 5 days with MDAMB-231 breast adenocarcinoma cell-conditioned medium (TCM). mRNAs isolated from TCM-treated and control cells were ampli®ed using 104 combinations of four 30 anchored T12VN primers and 26 `random' 10mers by RTPCR and the products examined on DNA sequencing gels. Autoradiography:Article Title: Mitochondrial DNA polymerase gamma is essential for mammalian embryogenesis. Article Snippet: For Southern blot analyses, 5 mg of genomic DNA was digested with Bgl I and blotted to Hybond-Cþ ( Amersham) membrane using standard procedures. .. The membranes were hybridized with a 2.7 kb probe spanning exons 11–14 (Fig. 1A and B) labelled with [ Polymerase Chain Reaction:Article Title: The Use of a 3895 bp Mitochondrial DNA Deletion as a Marker for Sunlight Exposure in Human Skin Article Snippet: To confirm the identity of the 375 bp PCR product, the DNA was sequenced using automated DNA sequencing (MWG Biotech, Ebersberg, Germany). .. Radioactive PCR analysis To detect low levels of the deletion generated by UVR, the PCR was carried out as described above but with addition of 3 mCi of [ Generated:Article Title: The Use of a 3895 bp Mitochondrial DNA Deletion as a Marker for Sunlight Exposure in Human Skin Article Snippet: To confirm the identity of the 375 bp PCR product, the DNA was sequenced using automated DNA sequencing (MWG Biotech, Ebersberg, Germany). .. Radioactive PCR analysis To detect low levels of the deletion generated by UVR, the PCR was carried out as described above but with addition of 3 mCi of [ |